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Suzanne Mertens

Atlanta GA

flow cytometry application scientist

Interests: clinical flow reporting, technical writing, instrument performance standardization, data normalization, GLP validated data analysis pipelines, new software feature requests, automated cluster gating, high-content and high-throughput analysis, new cytometers and sorters, clinical trials management, new assay qualification/validation, QA testing.

Recent Activity

Big Trouble with Little Things

"See? That was nothing. But that's how it always begins. Very small." - Egg Shen When I want to confirm the low-end resolution of a flow cytometer, the size standards I use come from Spherotech, Specifically, catalog # NFPPS-52-4K on... Continue reading

Posted Feb 23, 2021 at flow cytometry

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Cleveland Clinic's flow protocols... TUNEL etc. Continue reading

Posted May 14, 2018 at flow cytometry

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Jablonski Diagrams

If I had a nickel for every time I needed to sketch out why things fluoresce... Care of Ken Hanson's presentation on "Introduction to Molecular Photophysics". Continue reading

Posted May 14, 2018 at flow cytometry

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The Janelia Fluor® dyes page has some quality org chem descriptions. Sirigen gives details about their FRET combinations. Continue reading

Posted May 14, 2018 at flow cytometry

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It should come as no surprise that the buffers used in sorting - ie sample buffer, and collection buffer - are quite different from one another AND from culture media. Scripps Sort Buffer... HEPES/EDTA/DNAse, polypropylene vs polystyrene ThermoFisher... Glutamax vs... Continue reading

Posted May 14, 2018 at flow cytometry

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Options to run CBCs on both human and mouse samples

I had a customer at UAB ask about options to run CBCs on both human and mouse samples... below are some options based on conversation on the Purdue Cytometry List. After posting this question, it also occurred to me that... Continue reading

Posted May 26, 2015 at flow cytometry

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NBS versus FCS versus BSA

NBS... Newborn Calf Serum... originates from 3 to 10 day old calves. Various suppliers claim that NCS contains a somewhat higher proportion of proteins and immunoglobulin, but fewer growth factors. FCS... Fetal Calf Serum... originates from bovine fetus. Supposedly contains... Continue reading

Posted Mar 15, 2012 at flow cytometry

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As per Maecker and Trotter... ...is defined as D/W, where D is the difference between positive and negative populations and W is equal to 2 SD of the negative population. And since FCS Express 3 does not offer SD*, we'll... Continue reading

Posted Mar 1, 2012 at flow cytometry

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CD117... aka C-Kit... cytokine receptor for stem cell factor (SCF). According to Canonico B, Felici C, Papa S.... c-kit expression in acute leukemia can be considered as an indicator of myeloid origin of tumor cells as it is rarely expressed... Continue reading

Posted Feb 28, 2012 at flow cytometry

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Fibroblast growth factor receptor 3... AKA CD333. R&D Systems seems to have the only clone specifically marketed for flow cytometry. Santa Cruz Biotech has SU5402 in RUO format. Chesi et al. posit that it's an oncogene for multiple myeloma: "Myeloma... Continue reading

Posted Feb 24, 2012 at flow cytometry

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LIMS with Flow Cytometry

There has been more and more talk over at FlowJo about various third-party system integrations - with LabArchives, Bioconductor/R via GenePattern, CytoGenie/Evertrieve and others. Here's a general list of more LIMS from CAP Today. News about LIMS systems can also... Continue reading

Posted Jul 22, 2011 at flow cytometry

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Biexponential Transformation settings

Mucking about in FlowJo is fun, especially when you can get immediate visual feedback from changing a transformation setting. For the below, I've chosen to plot only in Pseudocolor and vary only one setting at a time in FlowJo Mac... Continue reading

Posted May 5, 2011 at flow cytometry

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Update on Reference Ranges

A new round of bead gating is ready, this time with gating directions updated for doublet discrimination. If you would like to participate, you can download the bead files here. To read the details in the original posting about this,... Continue reading

Posted May 2, 2011 at flow cytometry

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Doublet discrimination gating

I found that using a positive control for douplets helps figure out the best gating method for doublet discrimination. Use this Doublet Discrimination on the FACSAria PDF to see plotting of Area, Height and Width for chicken erythrocyte nuclei from... Continue reading

Posted Apr 22, 2011 at flow cytometry

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Who to talk to about Assay Validation

Julie Wilkinson at Immunosite Technologies. David Favre at National Immune Monitoring. Continue reading

Posted Mar 31, 2011 at flow cytometry

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Reference ranges for clustering

In a collaborative effort with FlowJo, Steve Perfetto, and Ryan Brinkman's group, I'm collecting statistics from manual gating on bead files. The pupose is to define a reference range of statistics that adequately describes the variation from one manual analyst... Continue reading

Posted Mar 20, 2011 at flow cytometry

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Conference list

I'll be either attending or at the FlowJo booth for the following upcoming conferences - come on by and let's talk flow. Northwest Regional Cytometry Meeting AACR International Society for Laboratory Hematology American Association of Immunologists CYTO 2011 International Society... Continue reading

Posted Mar 3, 2011 at flow cytometry

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NIH on-campus dates

I'll be in the DC area on the following dates. Send me an email to my FJ account if you want to meet up. 8 - 11 March 26-29 April 17-19 May 21-22 June Continue reading

Posted Mar 3, 2011 at flow cytometry

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Alice Vines throws down calcium flux measurements from an Accuri C6, plotted in Kaluza. The time plots in this tech note are shedazzley. Continue reading

Posted Feb 12, 2011 at flow cytometry

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Julie Bruhn was kind enough to send me a few articles on optimized flow panels. Mario Roederer and Attila Tarnok announced the new OMIP format in Cytometry Part A. The first two, OMIP-001 and OMIP-002, are on my favorite bit... Continue reading

Posted Jan 15, 2011 at flow cytometry

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Suzanne Mertens has shared their blog flow cytometry

Jan 15, 2011

Suzanne Mertens is now following The Typepad Team

Mar 15, 2010

Thanks David. I can't replicate your error, but I can email the PowerPoint to you.

Commented Aug 15, 2006 on QC for LSRII at flow cytometry

Go ahead Maciej - link it up. Noah, you're not the only one with that bright idea. Hit PubMed and check out what the Herzenberg lab has recently published.

Commented Aug 15, 2006 on Using FlowJo to calculate compensation at flow cytometry

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